Research Article |
Corresponding author: Jordan Golubov ( jgolubov@gmail.com ) Academic editor: Renate Wesselingh
© 2023 Oscar Sandino Guerrero-Eloisa, Jordan Golubov, María C. Mandujano, Pedro Luis Valverde.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Guerrero-Eloisa OS, Golubov J, Mandujano MC, Valverde PL (2023) The reproductive traits that contribute to the invasive success of Mediterranean onionweed (Asphodelus fistulosus). Plant Ecology and Evolution 156(2): 201-214. https://doi.org/10.5091/plecevo.89362
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Background and aims – Understanding the traits that lead to the invasion potential of invasive alien species (IAS) provides insight for their management. The reproductive traits of IAS help us understand the mechanisms that allow for their invasive potential, and colonization into new ranges. Asphodelus fistulosus is a native Mediterranean species commonly found invading Australia, South-East Asia, South Africa, and North America.
Materials and methods – Two populations of A. fistulosus in the Chihuahuan Desert were monitored for reproductive phenology. Floral visitors and their behaviour were described, and we assessed the breeding system through floral morphological characters and the mating system in controlled pollination experiments.
Key results – Reproductive phenology showed continuous reproduction throughout the year. Floral morphology suggested a facultative autogamous breeding system, but the mating system was mixed with autonomous selfing. Flowers lasted one day, with anthesis lasting 11 h. Floral visitors of A. fistulosus consisted of a variety of taxa including species of Coleoptera, Hymenoptera, and Lepidoptera, the exotic Apis mellifera being the most frequent visitor.
Conclusions – The reproductive traits of A. fistulosus in the invaded range provide the biological potential for further invasion. The continuous production of reproductive structures attracts many diverse pollinators, and the autonomous self-pollination implies that a single plant has the potential to develop a new population, which makes the control of this IAS a global challenge.
breeding and mating system, floral visitors, invasive alien species, phenology
Current severe environmental threats are brought about by changes in land use, climate change, and invasive alien species (IAS;
Variation in mating and breeding systems of invasive plant species is broad (
Seed output in IAS is thought to be high (
Asphodelus fistulosus L. (Xanthorrhoeaceae) is a native herb from the south of Europe, found in the Mediterranean basin and Macaronesian region (
The purpose of this study was threefold: (1) describe the flowering phenology of A. fistulosus at two invaded sites in the Chihuahuan Desert, (2) identify floral visitors and how these change over floral anthesis, and (3) describe the breeding system using morphological floral characters and define the mating system through field-controlled pollination experiments to assess how these traits can favour the invasion potential of A. fistulosus in Mexico.
Field work was carried out at two sites in the Southern Chihuahuan Desert. The first site QRO was located close to Cadereyta de Montes, Queretaro, Mexico (20°47’24”N, 99°43’27”W), the type of soil is calcareous (
A patch of vegetation invaded by A. fistulosus was identified in September 2018 at each site. We followed all individuals of A. fistulosus within 1 × 1 m plots at each site (N = 17 plots in QRO and N = 10 plots in SLP). The number of plots was based on the abundance of A. fistulosus individuals at each site, taking the loss of individuals over the period as well as availability of reproductive individuals into account. Sample size started at QRO = 1015 and SLP = 999 individuals and diminished over the study period to QRO = 791 and SLP = 856 individuals. All plants of A. fistulosus in each plot were tagged, mapped, and the frequency of their phenological phase recorded every two months from September 2018 to July 2019. We followed three phenophases (floral buds, flowers, and fruits) that were analyzed with circular statistics using a Rayleigh test to identify deviations from a uniform distribution for each site and a Watson-Williams two-sample test (U2) to test for differences in phenophases between sites. All circular statistics by phenophases were run on Oriana v.4.0 (
Meteorological data were obtained (average temperature and average precipitation) online (www.wunderground.com) from the nearest weather station (IQUERETA 15) for the study period. We correlated these environmental variables with the phenology observed in QRO.
Observation data from photographs that could identify the phenological states (floral buds, flowers, and fruits) of A. fistulosus in the citizen science portal iNaturalist Mexico (
Observations of floral visitors were carried out in February 2019 at QRO and March 2019 in SLP. At each site, five flowers of 15 individuals of A. fistulosus in each of three plots were monitored for visitor activities. Each plot was monitored by one observer during anthesis (225 flowers in QRO, five observers, 15 flowers in three plots and 180 flowers in SLP, four observers, 15 flowers in three plots). Forty-five-minute observation periods were made at each site from 07h00 to 18h00 with 15-minute breaks for each period of observation. The identity (species or morphospecies) of the visitor, activity (catalogued as pollen or nectar collection), and time of observation were recorded for each visit (
Anthesis was followed in QRO (February 2019) and SLP (March 2019) in three plots. Fifteen flowers of three different individuals were followed for corolla aperture and measured with a digital calliper (to the nearest 0.05 mm) in 15-minute intervals from 07h00 to 18h00. Stigmas were considered receptive by a change in colouration (light pink to dark pink) and moisture on the stigmatic surface, while anther dehiscence was detected with the presence of pollen. Circular statistics were used to describe floral behaviour where the mean angle (μ) represented mean time of aperture and the vector (r) the concentration of frequency around the mean through a Rayleigh test (
Accumulated nectar production was obtained from 30 flowers using microcapillary tubes (1 μl) on flowers bagged before anthesis (09h00) and sampled at 18h00. Nectar concentration was estimated with a field refractometer (Atago mod. N-1α).
The mating system was determined through controlled pollination experiments in the QRO population during February 2020. The same experiment was established at SLP but was soon vandalized. One flower of each of 50 individuals (blocks) was assigned to one of the following seven treatments: (1) control: flowers were tagged and exposed to natural pollination; (2) supplementary pollen: to evaluate pollinator limitation, additional pollen from other individuals was deposited on exposed flowers; (3) artificial self-pollination: flowers were bagged with bridal cloth before anthesis, manually pollinated with self-pollen and rebagged; (4) autonomous self-pollination: flowers were bagged with bridal cloth before anthesis without further manipulation; (5) artificial cross pollination: flowers were bagged with bridal cloth before anthesis, emasculated at the onset of anthesis, pollinated manually with pollen from other individuals and rebagged; (6) natural cross pollination (cross pollination control): flowers were bagged before anthesis not emasculated at the onset of anthesis, pollinated manually with pollen from other individuals; (7) geitonogamy: flowers of the same plant were bagged and manually pollinated with pollen from flowers of the same individual. An agamospermy treatment was attempted, but self-pollen contamination precluded further evaluation. Fruit set was recorded three weeks after the onset of the pollination treatments. Results of the pollination experiments were analyzed through GLM with a binomial error distribution in JMP® v.16.0.0 (
Floral morphological traits were taken to determine the breeding system. The out-crossing index (OCI) and pollen/ovule ratio (P/O;
Reproductive phenophases at the two studied populations in the Chihuahuan desert (QRO and SLP) were studied throughout the 11-month study period (Table
Circular plots of the phenophases of the reproductive structures (floral buds, flowers, and fruits) of Asphodelus fistulosus. Upper plots correspond to the QRO site (A–C) and lower plots to the SLP site (D–F). Bars represent the frequency of each phenophase, the arrow the magnitude of the mean vector (r). Green lines indicate the start and red lines the finish of phenology observations at both sites.
Results of the circular statistical analysis for the occurrence of seasonality in the reproductive phenological patterns of Asphodelus fistulosus observed in Cadereyta, QRO, and Guadalcazar, SLP. The Rayleigh test assesses deviations from a uniform distribution.
Site | Phenophase | Z test | Mean vector (r) | Rayleigh test (p) |
Cadereyta (QRO) | Floral buds | 13.68 | 0.25 | < 0.001 |
Flower | 1.67 | 0.17 | = 0.188 | |
Fruits | 160.24 | 0.52 | < 0.001 | |
Guadalcazar (SLP) | Floral buds | 46.54 | 0.35 | < 0.001 |
Flower | 20.09 | 0.35 | < 0.001 | |
Fruits | 137.64 | 0.48 | < 0.001 |
The phenology results suggest that floral buds, flowers, and fruits are influenced by at least one environmental variable in QRO. Floral buds showed a positive correlation with precipitation (r = 0.97, p < 0.001), flowers were positively correlated with temperature (r = 0.69, p < 0.001), and fruits were correlated with precipitation (r = 0.30, p < 0.001) and temperature (r = 0.38, p < 0.001).
Phenophases observed on the citizen science platform (iNaturalist Mexico) showed all reproductive phenophases present year-round. QRO showed a synchronous production of buds, flowers, and fruits with a peak in winter (Fig.
Circular plots of the phenophases of the reproductive structures (floral buds, flowers, and fruits) of Asphodelus fistulosus using Citizen Science observations in Mexico. Upper plots correspond to the Queretaro State (A–C) and lower plots to San Luis Potosí State (D–F). Bars represent the frequency of each phenophases, the arrows the magnitude of the mean vector (r).
Results of the circular statistical analysis for the occurrence of seasonality in the reproductive phenological patterns of Asphodelus fistulosus observed in Queretaro and San Luis Potosí States using iNaturalist observations from 2013 to 2022. The Rayleigh test assesses deviations from a uniform distribution.
State | Phenophase | Z test | Mean vector (r) | Rayleigh test (p) |
Queretaro | Floral buds | 7.3 | 0.46 | < 0.001 |
Flower | 7.78 | 0.48 | < 0.001 | |
Fruits | 4.85 | 0.43 | < 0.001 | |
San Luis Potosí | Floral buds | 3.32 | 0.19 | 0.036 |
Flower | 1.56 | 0.12 | 0.209 | |
Fruits | 0.14 | 0.04 | 0.867 |
During the 10 observation periods at each site, 13 species of floral visitors were identified in QRO belonging to Hymenoptera (six spp.), Coleoptera (one sp.), and Lepidoptera (six spp.), and eight species were registered in SLP belonging to Formicidae (one sp.), Apidae (two spp.), Halictidae (one sp.), and Lepidoptera (four spp.) (Table
Floral visitors, activity (N = nectar, P = pollen), origin (NA = North America, A = America, E = exotic), and time spent on flowers (seconds) of Asphodelus fistulosus at two sites in the southern Chihuahuan Desert (QRO and SLP).
Order | Family | Genus | Species | Activity | Mean time spent in activity (s) | Site | Origin |
Coleoptera | Melyridae | Trichochrous | – | N, P | 113 | QRO | NA |
Hymenoptera | Apidae | Ashmeadiella | – | N | 3 | QRO | A |
Hymenoptera | Apidae | – | – | N | 8 | QRO | – |
15 | SLP | ||||||
Hymenoptera | Apidae | Ceratina | – | N, P | 20 | QRO | – |
Hymenoptera | Apidae | Apis | mellifera | N, P | 11 | QRO | E |
16 | SLP | ||||||
Hymenoptera | Formicidae | – | – | N, P | 15 | SLP | – |
Hymenoptera | Halictidae | Lasioglossum | – | N, P | 11 | QRO | – |
7 | SLP | ||||||
Hymenoptera | Halictidae | – | – | N,P | 12 | QRO | – |
Lepidoptera | – | – | – | N | 7 | SLP | – |
Lepidoptera | Geometridae | Metanema | inatomaria | N | 6 | SLP | A |
Lepidoptera | Hesperiidae | Copaeodes | minima | N | 7 | QRO | A |
Lepidoptera | Lycaenidae | Echinargus | isola | N | 27 | SLP | NA |
Lepidoptera | Lycaenidae | Hemiargus | ceraunus | N | 13 | QRO | A |
Lepidoptera | Lycaenidae | Leptotes | marina | N | 8 | QRO | NA |
Lepidoptera | Nymphalidae | Anthanassa | texana | N | 4 | QRO | NA |
Lepidoptera | Nymphalidae | Agraulis | vanillae | N | 6 | SLP | A |
Lepidoptera | Nymphalidae | Texola | elada | N | 3 | QRO | NA |
Lepidoptera | Pieridae | Catasticta | nimbice | N | 10 | QRO | A |
The activities (collecting nectar or pollen) were divided into the Lepidoptera that exclusively collected nectar, while the Hymenoptera collected both nectar and pollen (Table
Asphodelus fistulosus produced fruits without pollinators, had the capacity for autonomous pollination, and was self-compatible. Pollination experiments showed high fruit set with no differences among treatments (χ2 = 9.17, d.f. = 6, p = 0.164), which indicates a mixed mating system. Floral morphometric data (Table
Floral morphological measurements (mean ± SE) of Asphodelus fistulosus (N = 65 flowers) from different individuals for each site (QRO and SLP).
Floral trait | QRO | SLP |
Perianth width (mm) | 16.77 ± 0.22 | 15.80 ± 0.21 |
Spatial separation of stamens-stigmas (mm) | 0.30 ± 0.12 | 0.16 ± 0.28 |
Stigma height (mm) | 6.24 ± 0.12 | 5.56 ± 0.05 |
Number of ovules | 6 | 6 |
Pollen grains per flower | 2304 ± 61 | 2268 ± 72 |
The P/O ratio was high (QRO = 384:1 and SLP = 378:1) and consistent with the out-crossing index (OCI) estimation for facultative autogamous species. When comparing autonomous pollination treatments (autonomous self-pollination, artificial self-pollination, and geitonogamy) vs cross pollination treatments (supplementary pollen, natural cross pollination, and artificial cross pollination), we found a small but significant difference (χ2 = 9.17, d.f. = 6, p = 0.028) in fruit set, which means that even though A. fistulosus is basically capable of both self- and cross pollination, autonomous pollination does have a slight advantage over cross pollination (Table
Fruit set of pollination experiments on Asphodelus fistulosus in QRO. N = sample size (number of flowers); mean ± SE for each treatment.
Pollination treatment | N | Fruit set |
Control | 40 | 0.85 ± 0.36 |
Supplementary pollen | 42 | 0.71 ± 0.45 |
Artificial self-pollination | 44 | 0.84 ± 0.36 |
Autonomous self-pollination | 40 | 0.82 ± 0.38 |
Artificial cross-pollination | 44 | 0.65 ± 0.47 |
Natural (control) cross-pollination | 43 | 0.67 ± 0.47 |
Geitonogamy | 41 | 0.75 ± 0.44 |
Reproductive traits in IAS are considered important components in their invasion potential (
A second component in the success of IAS that is reflected in phenological events is phenotypic plasticity. At the study sites in the Chihuahuan Desert, A. fistulosus reproduction peaked during two different seasons (summer in QRO and spring in SLP), while data from other countries suggest flowering peaks in summer in the USA (
As a possible consequence of extended flowering periods, there is also an enhanced attraction towards floral visitors (
Not only is the length of the flowering period relevant for IAS success but also floral longevity, because these determine on one hand the seasonality in the reproductive periods and on the other the availability of resources at any given time (
The role played by mating and breeding systems spurred Baker’s law, according to which selfing species would be better colonizers (
In addition to the number of reproductive traits found in A. fistulosus that very likely increase its invasive success, the establishment of A. fistulosus in areas that present a high disturbance regime also contributes to their success (
All reproductive phenophases of the species in both populations were found throughout the year, providing continuous availability of resources for floral visitors. However, flower and fruit production peaks differed between populations, suggesting that reproductive phenology responded to local conditions. The large number of flowers favoured the presence of native visitors, which ranged from nectarivorous species of Lepidoptera, species of native bees (e.g., Lasioglossum sp. and Ceratina sp.), to exotic bees (Apis mellifera) that collect pollen and nectar. Our evidence supports Baker’s law that self-pollinated species would be better colonizers. Furthermore, the mixed mating system of A. fistulosus guarantees variable offspring and dispersal to new habitats, and through autonomous pollination it generates progeny without the need for another individual. The invasive potential of onionweed within the Chihuahuan Desert is favoured by its mating system and phenological plasticity facilitating its expansion to other areas, prompting an urgent need to establish plans for its control.
The first author is a student of the PhD Program Doctorado en Ciencias Biológicas y de la Salud - Universidad Autónoma Metropolitana Xochimilco (UAM-X), and the paper is part of his dissertation in partial fulfilment of the requirements for the graduate program. This research was funded by Consejo Nacional de Ciencia y Tecnología (CONACyT) that awarded a scholarship to O.S.G-E. (815924). Financial and logistical support was provided by the Instituto de Ecología, UNAM, SEP-CONACyT 221362 and the GEF 00089333 project “Enhancing National Capacities to manage Invasive Alien Species (IAS) by implementing the National Strategy on IAS” to M.C.M. and J.G. We thank Mariana Rojas-Arechiga for logistical support and comments. Isabel Briseño Sánchez, Diana Cárdenas Ramos, Esteban Munguía Soto, Omar Díaz Segura, José Aranda Pineda, Jessica Reyes Tovar, Gerardo Manzanares Villasana, and Linda Martínez Ramos for field assistance. Two anonymous reviewers and the associate editor provided thoughtful insight and their suggestions helped improve the manuscript.