Plant Ecology and Evolution 152(2): 188-202, doi: 10.5091/plecevo.2019.1623
Haslea nusantara (Bacillariophyceae), a new blue diatom from the Java Sea, Indonesia: morphology, biometry and molecular characterization
expand article infoFiddy Semba Prasetiya‡, Romain Gastineau‡, Michel Poulin§, Claude Lemieux|, Monique Turmel|, Agung Dhamar Syakti, Yann Hardivillier‡, Ita Widowati#, Yenny Risjani¤, Iskandar Iskandar«, Toto Subroto», Charlotte Falaise‡, Sulastri Arsad¤, Ikha Safitri˄, Jean-Luc Mouget‡, Vincent Leignel‡
‡ FR CNRS 3473 IUML, Mer-Molécules-Santé (MMS), Le Mans Université, Avenue Olivier Messiaen, 72085 Le Mans CEDEX 9, France§ Division Recherche et collections, Musée canadien de la nature, C.P. 3443, Succursale D, Ottawa, Ontario, K1P 6P4, Canada| Institut de Biologie Intégrative et des Systèmes, Département de Biochimie, de Microbiologie et de Bio-Informatique, Université Laval, Québec, Canada¶ Fisheries and Marine Sciences Department, Jenderal Soedirman University, Kampus Perikanan Unsoed Karangwangkal, Jl dr. Suparno, Purwokerto 53123, Indonesia# Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. H. Soedharto,SH, Tembalang, Semarang 50275, Indonesia¤ Faculty of Fisheries and Marine Sciences, University of Brawijaya, Jalan Veteran, Ketawanggede, Malang 65145, Indonesia« Marine Science Department, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM.21, Jatinangor 45363, Indonesia» Research Center of Biotechnology and Bioinformatics, Jl. Raya Bandung Sumedang KM.21, Jatinangor 45363, Indonesia˄ Marine Science Department, Faculty of Mathematics and Natural Sciences Universitas Tanjungpura, Jl. Prof. Dr. H. Hadari Nawawi, Pontianak, Kalimantan Barat 78124, Indonesia

Corresponding author: Fiddy Prasetiya ( fiddy.semba.prasetiya@unpad.ac.id )

© Fiddy Semba Prasetiya, Romain Gastineau, Michel Poulin, Claude Lemieux, Monique Turmel, Agung Dhamar Syakti, Yann Hardivillier, Ita Widowati, Yenny Risjani, Iskandar Iskandar, Toto Subroto, Charlotte Falaise, Sulastri Arsad, Ikha Safitri, Jean-Luc Mouget, Vincent Leignel.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Prasetiya FS, Gastineau R, Poulin M, Lemieux C, Turmel M, Syakti AD, Hardivillier Y, Widowati I, Risjani Y, Iskandar I, Subroto T, Falaise C, Arsad S, Safitri I, Mouget J-L, Leignel V (2019) Haslea nusantara (Bacillariophyceae), a new blue diatom from the Java Sea, Indonesia: morphology, biometry and molecular characterization. Plant Ecology and Evolution 152(2): 188-202. https://doi.org/10.5091/plecevo.2019.1623
Open Access
Abstract

Background and aims – The present study aims to describe a new species of pennate blue diatom from the genus Haslea, H. nusantara sp. nov., collected from Semak Daun Island, the Seribu Archipelago, in Indonesian marine waters.

Methods – Assessment for species identification was conducted using light microscopy, Scanning Electron Microscopy and molecular techniques. The morphological characteristics of H. nusantara have been described, illustrated and compared to other morphologically similar blue Haslea taxa, distributed worldwide. Additionally, molecular characterization was achieved by sequencing plastidial and mitochondrial genomes.

Key results – This new species, named Haslea nusantara, cannot be discriminated by its morphology (stria density) but it is characterized by its gene sequences (rbcL chloroplast gene and cox1 mitochondrial gene). Moreover, it differentiates from other blue Haslea species by the presence of a thin central bar, which has been previously reported in non-blue species like H. pseudostrearia. The complete mitochondrion (36,288 basepairs, bp) and plastid (120,448 bp) genomes of H. nusantara were sequenced and the gene arrangements were compared with other diatom genomes. Phylogeny analyses established using rbcL indicated that H. nusantara is included in the blue Haslea cluster and close to a blue Haslea sp. found in Canary Islands (H. silbo sp. ined.).

Conclusions – All investigations carried out in this study show that H. nusantara is a new blue-pigmented species, which belongs to the blue Haslea clade, with an exceptional geographic distribution in the Southern Hemisphere.

Keywords
Indonesia, Bacillariophyceae, blue diatoms, Haslea, molecular phylogeny, genomic study

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