Genetic diversity of the extremely rare Habenaria dentata and the rare Habenaria linearifolia (Orchidaceae) in South Korea: implications for population history and conservation

Mi Yoon Chung; Hoa Thi Quynh Le; Sungwon Son; Huai Zhen Tian; Myong Gi Chung

doi:10.5091/plecevo.2018.1366

Plant Ecology and Evolution 151(1): 48-60, doi: 10.5091/plecevo.2018.1366

Genetic diversity of the extremely rare Habenaria dentata and the rare Habenaria linearifolia (Orchidaceae) in South Korea: implications for population history and conservation

Mi Yoon Chung^‡, Hoa Thi Quynh Le^‡, Sungwon Son^§, Huai Zhen Tian^|, Myong Gi Chung^‡

‡ Division of Life Science and the Research Institute of Natural Science, Gyeongsang National University, Jinju 52828, Republic of Korea§ Plant Conservation Division, Korea National Arboretum, Pocheon 11186, Republic of Korea| School of Life Sciences, East China Normal University, Shanghai 200241, China

Corresponding author: Myong Gi Chung ( mgchung@gnu.ac.kr )

Citation: Chung MY, Quynh Le HT, Son S, Tian HZ, Chung MG (2018) Genetic diversity of the extremely rare Habenaria dentata and the rare Habenaria linearifolia (Orchidaceae) in South Korea: implications for population history and conservation. Plant Ecology and Evolution 151(1): 48-60. https://doi.org/10.5091/plecevo.2018.1366

Abstract

Background and aims – Since historical events often leave an indelible mark on levels of genetic diversity of plant populations, one may indirectly infer their evolutionary history with the help of current patterns of genetic diversity. The terrestrial orchid Habenaria dentata, an element of warm-temperate/subtropical vegetation, reaches its northernmost limits in the Korean Peninsula, and thus it is extremely rare there. As H. dentata was absent from the Peninsula during the Last Glacial Maximum (LGM), it is likely to be of post-glacial origin having arrived from either a single refugium or multiple refugia. However, its rare, temperate/boreal congener H. linearifolia might have persisted in situ in either macrorefugia or microrefugia on the Peninsula during the LGM.

Methods – To test which hypothesis is most appropriate for each species, we investigated levels of allozyme-based (17 loci) genetic diversity and population genetic structure in the two only known populations of H. dentata and in 12 populations of H. linearifolia.

Key results – No allozyme diversity was found in H. dentata (H_e = 0.000), whereas H. linearifolia exhibited low within-population variation (H_e = 0.060) and high among-population differentiation (F_ST = 0.237). We found little association between populations in relation to their geographic location; several populations presented individuals belonging to different clusters.

Conclusions – Our results suggest that H. dentata likely originated from a single ancestral population (perhaps from southern Japan or southern China) through post-glacial dispersal, whereas H. linearifolia probably survived the LGM in situ in microrefugia situated at low to mid-elevated regions. We further suggest that separate conservation strategies for each species should be employed, given that the two taxa have different ecological and demographic traits and harbour different levels of genetic diversity.

Keywords

conservation, genetic diversity, Habenaria, historical events, population history

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