Prior to collection, we secured a permit from the Department of Environment and Natural Resources – Biodiversity Management Bureau (DENR GP Nos. 307 and 312). A total of 26 Philippine Amorphophallus specimens were collected across the archipelago from 2023 to 2024, following established protocols for field collection. Sampling was mostly from Luzon (17 specimens), followed by Visayas (5 specimens) and Palawan (4 specimens), representing approximately 50% of the recognized Philippine species. Specimens were identified to species based on diagnostic phenotypic characteristics using taxonomic keys and literature, as well as expert identification by regional experts. Figure 1 shows photographs of the collected species of Amorphophallus with available inflorescences. Specimens illustrated in Fig. 1A–J were collected in situ from various regions, while Fig. 1K was a cultivated specimen from Boracay. Supplementary material 1 lists all Philippine collections of Amorphophallus included in the study along with their corresponding voucher numbers deposited at the University of Santo Tomas Herbarium (USTH). Field-collected tissue was immediately preserved in silica gel for subsequent molecular analysis.

Of the 26 sampled collections, 12 were successfully identified to the species level, two of which represented the same species collected from different localities. However, not all identified collections included floral parts. Additionally, two collections bearing reproductive structures could not be identified to the species level because of their morphologically ambiguous floral characteristics, which deviated markedly from those of known species; therefore, recollection and further morphological analysis are needed to confirm their identities. The remaining 12 collections were sterile, further complicating identification, and the molecular markers employed in this study were insufficient to resolve their taxonomic placement.

Total genomic DNA was extracted from silica-dried leaf tissue using the Qiagen DNeasy Plant Mini Kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol with minor modifications to optimize DNA yield and purity. DNA quality and concentration were assessed using nanodrop spectrophotometry and agarose gel electrophoresis. The extracted DNA was stored at -20°C until PCR amplification.

Three DNA regions were amplified using established primer sets and PCR protocols (Suppl. material 2). PCR reactions were performed in 25 μL volumes containing 12.5 μL GoTaq Green Master Mix (Promega), 1.0 μL each of forward and reverse primers (10 μM), 1.0 μL template DNA (10–50 ng/μL), and 9.5 μL nuclease-free water. PCR products were visualized on 1.5% agarose gels stained with ethidium bromide under UV transillumination.

Amplified products were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The purified amplicons were sent to Macrogen Inc. (Seoul, South Korea) for bidirectional Sanger sequencing using the same primers employed in PCR amplification. Raw sequence chromatograms were manually inspected and edited using Geneious Prime 2024.0.5 (https://www.geneious.com/). Forward and reverse sequences were assembled into consensus sequences, and ambiguous bases were resolved by visually inspecting the chromatogram peaks. BLASTn searches were conducted against the NCBI GenBank database to verify sequence identity and to detect potential contamination.

In addition to the newly sequenced Philippine taxa (Suppl. material 3), we incorporated 158 Amorphophallus taxa with taxonomic coverage based on previous phylogenetic studies (Grob et al. 2004; Sedayu et al. 2010; Claudel et al. 2017; Wong et al. 2022). Three outgroup taxa were selected from related Araceae genera, Anchomanes difformis (Blume) Engl., Gonatopus angustus N.E.Br., and Hapaline sp., following standard practices in Amorphophallus phylogenetics.

Sequences for each marker were aligned separately using MAFFT v.7.490 (Katoh and Standley 2013) with default parameters. Alignments were visually inspected and manually adjusted in Geneious Prime where necessary to optimize positional homology. For ITS1, ambiguously aligned regions were excluded from the phylogenetic analyses. Sequence characteristics, including alignment length, number of variable sites, parsimony informative characters, and conserved sites, were calculated using MEGA v.12 (Kumar et al. 2024).

Phylogenetic analyses were conducted for each marker independently, as well as for the combined plastid + nuclear dataset. For each dataset, optimal nucleotide substitution models were chosen using MrModeltest2 under the Akaike Information Criterion (AIC) (Nylander 2004). Maximum Likelihood (ML) analyses were conducted based on the GTR+Γ+I model using RAxML-NG v.1.1 (Kozlov et al. 2019) with 1,000 bootstrap replicates. ML searches employed 20 random starting trees to ensure a thorough exploration of the tree space. Bayesian inference (BI) was performed using MrBayes v.3.2 under the GTR+I+Γ model with four Markov Chain Monte Carlo (MCMC) chains (three heated, one cold) (Ronquist et al. 2012). Each run consisted of approximately 10 million generations, with sampling every 1,000th generation across two independent runs. Convergence was assessed by monitoring the standard deviation of split frequencies (< 0.01) and examining potential scale reduction factors (approaching 1.0). The first 25% of trees were discarded as burn-in, and the remaining trees were used to construct 50% majority-rule consensus trees with posterior probabilities (PP).

Prior to combining the datasets, individual gene trees were assessed through visual inspection for strongly supported topological conflicts (bootstrap support [BS] ≥ 70%, PP ≥ 0.95). No strongly supported incongruence was detected; therefore, the datasets were concatenated, and the combined matrix was partitioned by gene region, allowing for separate substitution models for each partition in both ML and BI analyses. Phylogenetic trees were visualized and edited using FigTree v.1.4.4 (Rambaut 2018) and Interactive Tree of Life (iTOL) (Letunic and Bork 2007). BS ≥ 70% and PP ≥ 0.95 were considered strong indicators of phylogenetic relationships.

The success of PCR amplification and sequencing varied for each of the markers. From the chloroplast markers, the rbcL region was successfully amplified for all 26 Amorphophallus specimens, while the matK marker yielded 18 specimens that produced high-quality sequences that were useful for phylogenetic analysis. The matK sequences of eight specimens were not obtained because of weak amplification and low-quality sequences, probably because of the degraded DNA template. For the ITS1 region, 19 specimens were successfully sequenced, but seven samples produced unreadable sequences because of suspected low DNA concentration or fungal contamination.

For rbcL, the closest matches were A. albus, A. hottae Bogner & Hett., A. paeoniifolius (Densst.) Nicolson, A. sumawongii (Bogner) Bogner & Mayo, and A. titanum (Becc.) Becc., indicating that the Philippine specimens are most likely affiliated with Southeast Asian species. For matK, the top matches were A. angulatus Hett. & A.Vogel, A. eburneus Bogner, A. palawanensis Bogner & Hett., A. paeoniifolius, and A. titanum. MNM05 collected from Palawan exhibited the highest similarity to A. natolii Hett., Wistuba, Amoroso, Medecilo & Claudel (99.89%), confirming a close affinity with this Palawan endemic.

The individual dataset consisting of ITS1 had 169 taxa and a total aligned length of 663 bp, with 333 variable sites (50.2%). Of these 333 variable sites, 208 were parsimony informative (31.4%). For the rbcL dataset, 187 taxa were included, consisting of 1,496 bp with 242 variable sites (16.2%) and 133 (8.9%) parsimony informative sites. The matK dataset contained 176 taxa and 1,850 bp, with 473 variable sites (25.6%) and 209 parsimony informative sites (11.2%). The combined plastid dataset (rbcL + matK) comprised 187 specimens and an aligned length of 3,355 bp, of which 699 bp (20.8%) were variable sites and 366 bp (10.9%) were parsimony-informative. The combined plastid and nuclear dataset comprised the same 187 specimens—158 Amorphophallus species and three outgroups from which sequences were retrieved from GenBank, combined with 26 Philippine Amorphophallus specimens, for which new sequences were generated in this study—with an aligned length of 3,945 bp, of which 1,030 bp (26.1%) were variable sites and 546 bp (13.8%) were parsimony-informative (Suppl. material 4).

ITS1 had the highest number of parsimony informative characters (31.4 %). This is indicative of rapid changes and a greater phylogenetic signal at lower taxonomic levels. In contrast, rbcL was more conserved at 8.9% of informative sites, which is consistent with its slow rate of evolution, whereas matK was slightly higher at 11.2%. This is congruent with other molecular studies on Araceae that suggest combined plastid-nuclear datasets provide complementary phylogenetic signals (Claudel et al. 2017; Haigh et al. 2023). The GTR+Γ+I model was selected as the optimal model for all datasets under AIC, indicating heterogeneous substitution rates with both among-site rate variation and a proportion of invariant sites.

Single-marker phylogenetic analyses have provided preliminary resolution of Amorphophallus relationships. The rbcL tree, although highly conserved with limited resolution at the species level, successfully recovered major subgeneric clades with moderate support. Philippine taxa clustered with Southeast Asian species, suggesting regional phylogenetic affinity. Several Philippine accessions were grouped with Bornean and Sumatran species, supporting biogeographic connections between Palawan-Philippine assemblages and Sundaland flora. However, low bootstrap support (< 70%) at many nodes indicated insufficient phylogenetic signal for confident species-level resolution using rbcL alone.

Separate phylogenetic analyses of the plastid (cpDNA) and nuclear ribosomal DNA (nrDNA) markers were conducted to assess their individual topologies prior to the dataset combination. Although the overall bootstrap (BS) and posterior probability (PP) support was generally low across most nodes, both datasets supported the monophyly of Amorphophallus, with the exception of the Bayesian tree derived from the ITS region, in which the outgroup Anchomanes difformis failed to resolve as sister to the genus (Suppl. materials 5, 6). Notable topological discrepancies that are poorly supported were observed between the cpDNA and nrDNA trees (Suppl. materials 5, 6); the nrDNA tree exhibited weaker resolution, particularly within subgeneric groups, and Philippine species were less clearly resolved than the cpDNA tree (Suppl. materials 5B, 6B). The combined cpDNA (rbcL + matK) tree provided comparatively better resolution (Suppl. material 5A). These discrepancies likely reflect the distinct evolutionary histories captured by plastid and nuclear genomes, which can yield incongruent phylogenetic signals owing to factors such as incomplete lineage sorting. Given these differences, the cpDNA and nrDNA datasets were combined to improve the resolution and provide a more comprehensive and robust phylogenetic framework for Amorphophallus.

The combined plastid and nuclear dataset provided substantially improved resolution and support compared with single-marker analyses. The resulting ML and BI trees were topologically congruent, with ML bootstrap (BS) values and BI posterior probabilities (PP) showing strong concordance. The combined phylogeny confirmed the monophyly of Amorphophallus (BS = 100%, PP = 1.00) and recovered major subgeneric divisions consistent with previous phylogenetic studies (Sedayu et al. 2010) (Fig. 2). However, Philippine taxa were distributed across multiple clades, confirming the polyphyly of Philippine Amorphophallus (Fig. 2).

Within A. subgenus Amorphophallus, a strongly supported Southeast Asian clade (BS = 89%, PP = 1.00) included most of the Philippine species (Fig. 2). Within this assemblage, the Paeoniifolius-Manta clade sensu Claudel et al. (2017) received strong support (BS = 95%, PP = 1.00) and encompassed several Philippine taxa including A. urceolatus Hett., A. samarensis, A. rostratus Hett., and multiple accessions of A. paeoniifolius from Bohol, Romblon, Nueva Ecija, and Bulacan, respectively (Fig. 2). These species share morphological synapomorphies, including sessile spadices, verruculate petiole ornamentation, and the absence of tuber offsets, corroborating phylogenetic grouping with morphological coherence. Interestingly, one specimen from Angat Watershed (originally identified as A. rostratus) was genetically indistinguishable from true A. rostratus, confirming species identity and extending its known distribution. Additionally, several accessions morphologically resembling A. paeoniifolius but from previously unreported localities (Nueva Ecija, San Miguel Bulacan) clustered within the A. paeoniifolius clade, suggesting potential cryptic diversity or intraspecific variation that warrants further investigation.

In comparison to most Philippine species that fall within A. subgenus Amorphophallus, two accessions from Palawan were assigned to A. subgenus Metandrium. Amorphophallus natolii and an unidentified specimen from Palawan (MNM05) formed a well-supported clade (BS = 96%, PP = 1.00) that was geographically isolated from all other Philippine members (Fig. 2). The isolation of A. subgenus Metandrium is supported by its morphology, characterized by stipitate spadices and distinctive tubers. The position of the Palawan clade indicates an independent colonization event in the Philippines, likely via southern routes connecting Palawan to Borneo, where A. subgenus Metandrium is more diverse. This result illustrates Palawan’s unique biogeographic history and its position as a land bridge between Sundaland and the Philippine archipelago.

Despite the increased resolution provided by the combined dataset, several polytomies remained unresolved, particularly within closely related taxa of the Paeoniifolius-Manta clade. Species-level relationships among A. paeoniifolius accessions from different islands showed limited resolution, suggesting rapid radiation or incomplete lineage sorting. Similarly, the placement of several Philippine endemics remained uncertain, with low statistical support for their sister-group relationships. These results highlight the limitations of three-marker approaches for fully resolving species-level phylogenies in rapidly diversifying lineages and underscore the need for phylogenomic studies using genome-scale datasets to resolve these relationships definitively.

The polyphyletic nature of Philippine Amorphophallus, as revealed by our molecular phylogenetic analyses, provides compelling evidence for multiple independent colonization events in the Philippines. The distribution of Philippine taxa across at least two distinct clades—the Paeoniifolius-Manta clade within A. subgenus Amorphophallus and the Palawan endemic clade within A. subgenus Metandrium—suggests that the Philippine archipelago was colonized by different biogeographic sources at different times. This result is broadly consistent with the subgeneric framework established by Claudel et al. (2017), who showed that the four main clades of Amorphophallus largely correspond to distinct geographic and climatic regions, with A. subgenus Amorphophallus centred on mainland Southeast Asia and A. subgenus Metandrium distributed primarily across the Malesian region, including Borneo. More recently, the phylogenomic study of Pouchon et al. (2023), which analysed 71 plastid genes from 36 species using genome skimming, provided a revised divergence timeline for the genus and a biogeographic scenario linking the origins of the major clades to climate dynamics and long-distance dispersal events near the Oligocene–Miocene transition (~23 Ma). The placement of Philippine taxa in two phylogenetically and geographically distinct subgenera, as recovered by our analyses, is consistent with this broader phylogenomic framework and suggests that the Philippine archipelago has served as a secondary colonization target from distinct source regions over a protracted timeframe. This pattern aligns with broader biogeographic hypotheses for Philippine flora, which postulate that the archipelago has functioned as a biodiversity sink, receiving taxa from multiple source regions, including mainland Southeast Asia, Sundaland, and Wallacea (Jones and Kennedy 2008). Within this context, it is noteworthy that Southeast Asia represents the most species-rich subregion of the genus, with Thailand harbouring the highest number of species owing to its favourable tropical and subtropical climatic conditions (Fontarum-Bulawin et al. 2025). The sporadic occurrence of Amorphophallus in some areas of its range further reflects the genus’ annual cycle of growth and dormancy, which, combined with specific environmental requirements including suitable vegetation cover, altitude, light availability, humidity, and temperature, can result in localized absences even within otherwise suitable biogeographic corridors.

The two colonization events inferred for Philippine Amorphophallus likely correspond to distinct biogeographic routes. The Paeoniifolius-Manta clade, which includes the majority of Philippine species, shows strong phylogenetic affinity with Southeast Asian mainland taxa, consistent with the biogeographic signal for A. subgenus Amorphophallus documented by both Claudel et al. (2017) and Pouchon et al. (2023), suggesting a northern colonization route via Taiwan or the Ryukyu Islands. In contrast, the Palawan endemic clade within A. subgenus Metandrium exhibits closer relationships with Bornean taxa, indicating a southern colonization route via the Sunda Shelf, possibly facilitated by reduced sea levels during Pleistocene glacial periods when Palawan was in closer proximity to Borneo. This Borneo-to-Philippines dispersal route is well documented in other Araceae: Nauheimer et al. (2012) inferred that the Philippines were reached from Borneo four to five times in Alocasia (Schott) G.Don during the Late Miocene to Early Pliocene, underscoring the permeability of this corridor for tropical understory monocots. Similar patterns of multiple, temporally staggered colonisations have been documented in other Philippine plant groups: Hughes et al. (2015) showed that Begonia sect. Baryandra A.DC. colonized the archipelago from western Malesia in the late Miocene, with Palawan and Luzon serving as early entry points, while Atkins et al. (2001) inferred Bornean origins for Philippine Cyrtandra J.R.Forst. & G.Forst. during the Pleistocene. Taken together, these studies indicate that Borneo and the Sunda Shelf have repeatedly served as source regions for Philippine plant diversity via the Palawan corridor, while independent dispersal events from the Asian mainland via northern island chains have contributed additional lineages, producing the complex, polyphyletic distributional patterns now observed in Amorphophallus and other genera.

The strongly supported Paeoniifolius-Manta clade recovered in our analysis represents a major Southeast Asian lineage first formally defined by Claudel et al. (2017), characterized by distinctive morphological synapomorphies, including sessile spadices, verruculate petiole ornamentation, and the absence of tuber offsets (Hetterscheid and Ittenbach 1996; Sedayu et al. 2010). Our results are consistent with Sedayu et al. (2010) and Claudel et al. (2017), who recognized a biogeographically distinct Southeast Asian clade spanning from India to the Philippines and encompassing Philippine endemics such as A. palawanensis and A. paeoniifolius. This affinity was further corroborated by Wong et al. (2022), who demonstrated that Philippine species, including A. longispathaceus Engl. & Gehrm., A. declinatus Hett., and A. palawanensis, cluster within the SEA clade alongside Bornean taxa, and by Pouchon et al. (2023), whose phylogenomic analysis placed A. palawanensis within this same clade, reinforcing the close evolutionary relationships between Philippine and broader Southeast Asian species of Amorphophallus. The inclusion of several Philippine taxa within this clade —A. urceolatus, A. samarensis, A. rostratus, and A. paeoniifolius—demonstrates that these species are phylogenetically nested within the Southeast Asian radiation (BS = 95%, PP = 1.00), a placement consistent with the regional affinities of this clade as established by Claudel et al. (2017) and further supported by Wong et al. (2022) for Bornean taxa. Although formal ancestral area reconstruction was beyond the scope of this study, this topological affinity suggests a Southeast Asian biogeographic origin for these Philippine lineages, a hypothesis that warrants explicit testing in future biogeographic analyses.

Within the Paeoniifolius-Manta clade, species-level relationships among Philippine taxa remain partially unresolved, with several polytomies indicating rapid radiation or insufficient phylogenetic signal from the three-marker dataset. This is not unexpected, given that similar polytomies have been reported in previous multi-locus studies of the genus (Sedayu et al. 2010; Claudel et al. 2017) and resolving them likely requires genome-scale data (Pouchon et al. 2023). The limited genetic differentiation among A. paeoniifolius accessions from different islands (Bohol, Romblon, Nueva Ecija, Bulacan) suggests either recent divergence or ongoing gene flow among island populations, consistent with the intraspecific morphological and palynological variation documented by Anil et al. (2023) across geographically disjunct morphotypes of this species. The wide distribution of A. paeoniifolius across island populations is further reflected in its status as the most frequently occurring species in the genus regionally (~12.31% of Southeast Asian occurrences), a pattern attributable in part to its widespread cultivation for its edible tuber and medicinal properties (Fontarum-Bulawin et al. 2025). However, the presence of morphologically distinct accessions within the A. paeoniifolius clade raises the possibility of cryptic species diversity, a concern further supported by the recent description of A. minimus R.Bustam., Claudel & M.N.Tamayo from Nueva Ecija (Bustamante et al. 2021) and A. calcicola M.N.Tamayo, Magtoto & Sumalinog from Bohol (Tamayo et al. 2021), both from localities represented in our sampling. These findings underscore the need for population genomic approaches and integrative taxonomic methods, combining morphological, molecular, and ecological data, to fully resolve species boundaries within this morphologically variable clade (Hetterscheid et al. 2020; Zhao et al. 2020).

The phylogenetic isolation of Palawan Amorphophallus within A. subgenus Metandrium provides strong molecular evidence for the biogeographic distinctiveness of Palawan flora, in agreement with the subgeneric framework of Claudel et al. (2017), who placed A. subgenus Metandrium primarily within the Malesian region, with Borneo as a centre of diversity. The well-supported clade comprising A. natolii and an unidentified Palawan specimen (MNM05; BS = 96%, PP = 1.00) is sister to other species of A. subgenus Metandrium from Borneo, corroborating the phylogenetic affinity between Palawan and Sundaland taxa inferred by Wong et al. (2022). This result is consistent with geological evidence indicating that Palawan formed part of the Asian continental shelf during Pleistocene glacial maxima, when sea levels were approximately 120 m lower than present, facilitating land connections between Palawan and northern Borneo (Voris 2000). The subsequent post-glacial rise in sea levels effectively isolated Palawan from Borneo, likely promoting the in situ differentiation of endemic lineages, including A. natolii, which was described from limestone cliffs at El Nido by Hetterscheid et al. (2012). Parallel patterns of Pleistocene vicariance and post-isolation divergence along the Palawan–Borneo corridor have been documented in other plant groups, including Alocasia (Nauheimer et al. 2012) and Cyrtandra (Atkins et al. 2001), affirming that this route has been a recurrent conduit for biotic exchange between Sundaland and the Philippines (Jones and Kennedy 2008).

The morphological distinctiveness of A. subgenus Metandrium, characterized by stipitate spadices and distinctive tuber morphology (Hetterscheid and Ittenbach 1996; Claudel et al. 2017), further reinforces the phylogenetic separation of the Palawan clade from all other Philippine Amorphophallus species. These morphological differences likely reflect adaptations to Palawan’s unique ecological conditions, including its extensive limestone substrates, seasonal rainfall regime, and distinct pollinator communities, conditions previously noted to promote localized endemism in A. natolii (Hetterscheid et al. 2012). The recognition of Palawan as a biogeographically distinct unit within the Philippines has important conservation implications, as the island harbours a disproportionately high proportion of endemic species relative to its area (Fontarum-Bulawin et al. 2025; Miranda et al. 2025). Our results reinforce the view that Palawan merits priority status in conservation planning for Amorphophallus and other aroid taxa, particularly given the ongoing threats to its limestone and lowland forest habitats.

Although our three-marker approach provided valuable insights into the broad phylogenetic structure of Philippine Amorphophallus, several relationships remained unresolved, particularly at the species level within the Paeoniifolius-Manta clade. The limited phylogenetic signal from plastid markers (rbcL, matK) and nuclear ribosomal ITS1 is consistent with recent findings in Araceae phylogenomics demonstrating that genome-scale datasets are necessary for resolving relationships among closely related species (Henriquez et al. 2014; Haigh et al. 2023; Zhao et al. 2023). Within Amorphophallus, Pouchon et al. (2023) demonstrated that analysing multiple plastid genes from a broad species sampling via genome skimming substantially improved subgeneric resolution compared to single- or few-gene approaches; however, they acknowledged persistent uncertainties at the species level, underscoring the inherent limitations of plastid-only or lightly sampled nuclear data. Similarly, whole-chloroplast genome analyses of individual species, such as A. konjac and A. albus, have contributed to resolving shallow-level placement (Li et al. 2024); however, fine-scale resolution of intrageneric diversity across the archipelago will require substantially broader taxon sampling with genome-scale markers.

Future phylogenomic studies on Philippine Amorphophallus should employ next-generation sequencing approaches, such as target enrichment of hundreds of low-copy nuclear genes or whole-genome resequencing, following the methodological frameworks successfully applied across Araceae by Haigh et al. (2023) and Zhao et al. (2023). Such approaches would enable more robust tests of biogeographic hypotheses, including the precise timing and directionality of colonization events, the role of Pleistocene glacial cycling in shaping island distributions (Esselstyn et al. 2009), and the mechanisms driving rapid diversification in island systems. Levin (2003) highlighted the role of cytoplasmic factors and plastid–nuclear conflict in plant speciation, and given the topological discrepancies observed between our cpDNA and nrDNA trees, explicit tests of reticulate evolution and hybridization using genomic data, as advocated by Mallet et al. (2016), would be particularly informative for groups with complex island biogeographic histories. Additionally, population genomic studies targeting widespread species, such as A. paeoniifolius, are needed to elucidate patterns of gene flow among island populations and to identify cryptic diversity potentially masked by morphological plasticity, as highlighted by the intraspecific variation documented across its range (Anil et al. 2023).

The phylogenetic results presented here have important implications for the taxonomy and species delimitation of Philippine Amorphophallus. The recovery of morphologically distinct accessions within the A. paeoniifolius clade suggests that current taxonomic treatments may underestimate species diversity within this group. Several specimens from previously unreported localities (Nueva Ecija, San Miguel, Bulacan) clustered within the A. paeoniifolius clade but exhibited subtle morphological differences in leaf shape, petiole ornamentation, and inflorescence size. These differences, combined with genetic differentiation, suggest the possibility of cryptic species that warrant further investigation through integrative taxonomic approaches combining morphology, molecular phylogenetics, and population genetics. That unrecognized diversity persists even in well-collected localities is consistent with the broader pattern of ongoing species discovery in the Philippine archipelago: Hetterscheid et al. (2020) described five new species from various Philippine islands in a single paper, and subsequent work has continued to reveal novelties, including A. minimus from montane forest in Nueva Ecija, Luzon, the same province from which our morphologically divergent specimens were collected (Bustamante et al. 2021), and A. calcicola from karst forest in Bohol, Central Visayas, closely related to A. longispathaceus and distinguished by a suite of floral characters (Tamayo et al. 2021). Most recently, A. samarensis was described from Samar Island Natural Park, Eastern Visayas, resembling A. calcicola in several vegetative and floral characters but distinguishable by leaf rachis morphology, stigma shape, and anther structure (Fontarum-Bulawin et al. 2024). The description of these new species from Bohol, Nueva Ecija, and Samar demonstrates that Philippine Amorphophallus diversity remains incompletely documented and validates our inference that morphological differentiation observed among our specimens from novel localities may reflect genuine taxonomic novelty rather than intraspecific variation.

Similarly, the unidentified Palawan specimen (MNM05), which clustered with A. natolii but showed morphological differences, may represent an undescribed species or a distinct population of A. natolii. Amorphophallus natolii, described from limestone cliffs at El Nido, is among several Amorphophallus species endemic to Palawan (Hetterscheid et al. 2012), and the island’s complex karst topography is known to harbour highly localized populations. These findings collectively highlight the need for continued field surveys, targeted collections from limestone and montane habitats, and integrative taxonomic revisions to document the full extent of Amorphophallus diversity in the Philippines.